Molecular dynamics analyses of CLDN15 pore size and charge selectivity.

Using cell culture and molecular dynamics simulations, we have clarified the role of amino acid D55 in the claudin-15 channel function. D55 is important for channel permeability and selectivity in both charge- and size-selective manners. This insight into the structural determinant of claudin cation channel function provides potential insight for designing approaches to alter the claudin channel function to benefit human health.