Alkyne/Ruthenium(II) Complex-Based Ratiometric Surface-Enhanced Raman Scattering Nanoprobe for In Vitro and Ex Vivo Tracking of Carbon Monoxide.
Xiaojie QinYanmei SiZhaoyang WuKe ZhangJishan LiYadong YinPublished in: Analytical chemistry (2019)
Here, we report a surface-enhanced Raman scattering (SERS) nanosensor for real-time ratiometric detection of carbon monoxide (CO) based on a ligand displacement mechanism. This nanoprobe consists of a gold-silver (Au-Ag) alloy nanoparticle core as the highly active SERS substrate, an alkyne/ruthenium(II) (alkyne/Ru(II)) complex immobilized on the surface as the CO-sensing element, and a porous silica shell to improve the stability and biocompatibility of the particle. Displacement of the alkyne ligand by CO results in a decrease of the alkyne vibrations and an increase of the metal carbonyl complex signals, thus allowing the effective ratiometric detection of CO in real-time. The great potential of this assay for CO detection is validated in clean buffer environments, live cells, and tissue slices.
Keyphrases
- sensitive detection
- loop mediated isothermal amplification
- living cells
- quantum dots
- label free
- fluorescent probe
- gold nanoparticles
- real time pcr
- energy transfer
- hydrogen peroxide
- high throughput
- single molecule
- raman spectroscopy
- nitric oxide
- climate change
- tissue engineering
- reduced graphene oxide
- human health
- magnetic nanoparticles