Removal of acenaphthene from water by Triton X-100-facilitated biochar-immobilized Pseudomonas aeruginosa .

By adding the nonionic surfactant Triton X-100 and using biochar as an immobilization carrier, a Triton X-100-facilitated biochar-immobilized Pseudomonas aeruginosa (TFBIP) material was prepared using the sorption method and was used to treat acenaphthene in water. The results showed that a low concentration of Triton X-100 simultaneously promoted the sorption capacity of the biochar and the degradation activity of P. aeruginosa , thereby significantly enhancing the removal of acenaphthene from water by the immobilized P. aeruginosa material. Compared with the control without Triton X-100, a low concentration of Triton X-100 significantly increased the acenaphthene removal rate by 20-50%. The optimal conditions for preparing the TFBIP were a loading time of 24 h, the use of a bacterial suspension with a concentration of OD 600 = 0.2, and a Triton X-100 concentration of 10 mg L -1 . The optimized TFBIP material could efficiently remove acenaphthene from water at temperatures of 10-50 °C, pH values of 4.5-10.5, and NaCl concentrations of up to 0.2 mol L -1 . The new TFBIP material can be used for the treatment of wastewater and may also be directly used for the remediation of soils contaminated with organic pollutants.