A Rebuilding-Free Nucleic Acid Detection Strategy Enables Ultrasensitive Genotyping, N-in-1 Logic Screening and Accurate Multiplex Assay of Dangerous Pathogens.
Huan LiHuiying LuYidan TangHuaning WangYao XiaoBingling LiPublished in: Angewandte Chemie (International ed. in English) (2022)
Sensitive, rapid and low-cost nucleic acid detection is critical for controlling infectious pathogens. Here, we develop a ready-to-use and multimodal detection based on a rebuilding-free, ultrasensitive and selective strategy named dual hairpin ligation-induced isothermal amplification pro (DHLApro). Taking influenza A, influenza B, MERS-CoV, SARS-CoV-2 as model targets, we demonstrate DHLApro provides ≈zM level ultra-sensitivity, being equaling to 0.45 copy/μL in original sample. By simply changing the recognition module, a set of DHLApro components can be applied to a new target without performance loss. Moreover, DHLApro innovatively allows flexible logic/multiplex assay using one set of primer, for example, the "N pathogens-in-1" OR gate screening and accurate multi-channel multiplex assay. Compared with traditional methods, the cost of this logic/multiplex assay has been largely reduced and the cross-interference between the multiple primer sets is also avoided.
Keyphrases
- nucleic acid
- high throughput
- real time pcr
- sars cov
- label free
- loop mediated isothermal amplification
- respiratory syndrome coronavirus
- low cost
- gram negative
- high resolution
- single cell
- gold nanoparticles
- antimicrobial resistance
- quantum dots
- oxidative stress
- diabetic rats
- molecularly imprinted
- genome wide
- dna methylation
- multidrug resistant
- solid phase extraction