ANTIBACTERIAL PROPERTY TESTING OF TWO SPECIES OF TROPICAL PLANT LASIANTHUS (RUBIACEAE).
Tiwtawat NapiroonHenrik BalslevSutee DuangjaiDuangchai SookchaloemKongkanda ChayamaritWichai SantimaleeworagunSrunya VajrodayaPublished in: The Southeast Asian journal of tropical medicine and public health (2018)
Two tropical plant species, Lasianthus pilosus and Lasianthus stipularis
are used in traditional medicine but there have been no published studies of the
extracts of these plants against bacteria. In this study, we aimed to determine
the antimicrobial activities of the above two plants against two gram-positive
and seven gram-negative bacterial strains to determine the potential of these
two plant species for possible antimicrobial drug development. The antibacterial
activities of the lipophilic extracts of these plants were evaluated by disk
diffusion and broth microdilution methods. The zone diameters and minimum
inhibitory concentrations (MIC) for these plant extracts exhibited their highest
antibacterial activity against Pseudomonas aeruginosa, followed by Staphylococcus
aureus and Acinetobacter baumannii, respectively. The MIC of these extracts against
P. aeruginosa (ATCC 37166 and ATCC 27853) varied from 50 to 200 μg/ml. Thin
layer chromatography and detection using different specific reagents revealed
the presence of terpenoids, phenolic compounds and iridoid. Cell lysis due to
the effect of the lipophilic extracts of these tested plants was demonstrated using
scanning electron microscopy. In conclusion, the bioactive compounds of these
plants should be studied further to develop potential antimicrobial agents.
Keyphrases
- staphylococcus aureus
- gram negative
- multidrug resistant
- acinetobacter baumannii
- pseudomonas aeruginosa
- electron microscopy
- drug resistant
- biofilm formation
- climate change
- single cell
- escherichia coli
- cystic fibrosis
- silver nanoparticles
- methicillin resistant staphylococcus aureus
- risk assessment
- systematic review
- human health
- tandem mass spectrometry
- cell wall
- liquid chromatography
- wound healing
- ms ms