Detection of Coxiella antibodies in ruminants using a SucB recombinant antigen.
Gianmarco FerraraBarbara ColittiGabriela Flores-RamirezUgo PagniniGiuseppe IovaneSergio RosatiSerena MontagnaroPublished in: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc (2023)
The detection of Coxiella burnetii in ruminants remains challenging despite the use of new technology and the accumulation of novel knowledge. Serology tools, the primary methods of infection surveillance in veterinary medicine, have limitations. We used recombinant antigen production to develop an ELISA based on the SucB protein, one of the major immunodominant antigens described in humans and laboratory animals. We produced the antigen successfully in an Escherichia coli heterologous system, confirmed by sequencing and mass spectrometry, and seen as a band of ~50 kDa in SDS-PAGE and on western blot analysis. We compared the performance of the recombinant ELISA with a commercial ELISA. We observed agreement of 83.5% and a substantial Cohen κ value of 0.67 in our pilot study.
Keyphrases
- escherichia coli
- mass spectrometry
- loop mediated isothermal amplification
- cell free
- monoclonal antibody
- healthcare
- public health
- single cell
- dendritic cells
- south africa
- immune response
- staphylococcus aureus
- cystic fibrosis
- heat shock protein
- high performance liquid chromatography
- amino acid
- binding protein
- multidrug resistant