Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under overexpression conditions.
Lizhi ZhouHai YuKaihang WangTingting ChenYue MaYang HuangJiajia LiLiqin LiuYuqian LiZhibo KongQingbing ZhengYingbin WangYing GuNingshao XiaShao-Wei LiPublished in: BMC genomics (2020)
The ER2566 strain was used by both the general scientific community and the biotechnology industry. Reannotation of the E. coli ER2566 strain not only improved the RefSeq data but uncovered a key site that might be involved in the transcription and translation of genes encoding the lactose operon repressor. We proposed that our pipeline might offer a universal method for the reannotation of other bacterial genomes with high speed and accuracy. This study might facilitate a better understanding of gene function for the ER2566 strain under external burden and provided more clues to engineer bacteria for biotechnological applications.
Keyphrases
- escherichia coli
- high speed
- genome wide
- endoplasmic reticulum
- estrogen receptor
- single cell
- breast cancer cells
- healthcare
- transcription factor
- rna seq
- gene expression
- atomic force microscopy
- copy number
- machine learning
- big data
- mass spectrometry
- klebsiella pneumoniae
- artificial intelligence
- deep learning
- candida albicans