Immediate and sensitive detection of sporulated Bacillus subtilis by microwave release and tandem mass spectrometry of dipicolinic acid.
L Edwin GonzalezLucas J SzalwinskiBrett M MarshJ Mitchell WellsRobert Graham CooksPublished in: The Analyst (2021)
Spore lysis of Bacillus species is achieved by brief (1 min) microwave irradiation while tandem mass spectrometry (MS/MS) allows identification of the characteristic spore marker, dipicolinic acid. This rapid measurement, made on 105-108 spores, has significant implications for biothreat recognition.
Keyphrases
- bacillus subtilis
- tandem mass spectrometry
- ultra high performance liquid chromatography
- sensitive detection
- high performance liquid chromatography
- liquid chromatography
- gas chromatography
- simultaneous determination
- ms ms
- loop mediated isothermal amplification
- solid phase extraction
- high resolution
- high resolution mass spectrometry
- liquid chromatography tandem mass spectrometry
- quantum dots
- mass spectrometry
- radiofrequency ablation
- genetic diversity