L-glutamic acid-g-poly hydroxyethyl methacrylate nanoparticles: acute and sub-acute toxicity and biodistribution potential in mice.
Buket BakanFatih OltuluYeliz YıldırımAltuğ YavaşoğluSinan AkgölNefise Ülkü Karabay YavaşoğluPublished in: Arhiv za higijenu rada i toksikologiju (2023)
The aim of this safety study in mice was to determine in vivo toxicity and biodistribution potential of a single and multiple doses of L-glutamic acid-g-p(HEMA) polymeric nanoparticles as a drug delivery system. The single dose did not cause any lethal effect, and its acute oral LD 50 was >2.000 mg/kg body weight (bw). Multiple doses (25, 50, or 100 mg/kg bw) given over 28 days resulted in no significant differences in body and relative organ weights compared to control. These results are supported by biochemical and histological findings. Moreover, nanoparticle exposure did not result in statistically significant differences in micronucleus counts in bone marrow cells compared to control. Nanoparticle distribution was time-dependent, and they reached the organs and even bone marrow by hour 6, as established by ex vivo imaging with the IVIS ® spectrum imaging system. In conclusion, L-glutamic acid-g-p(HEMA) polymeric nanoparticles appear biocompatible and have a potential use as a drug delivery system.
Keyphrases
- bone marrow
- liver failure
- body weight
- respiratory failure
- drug induced
- drug delivery
- high resolution
- aortic dissection
- mesenchymal stem cells
- drug release
- induced apoptosis
- oxidative stress
- human health
- blood pressure
- type diabetes
- high fat diet induced
- cancer therapy
- hepatitis b virus
- metabolic syndrome
- computed tomography
- intensive care unit
- cell proliferation
- cell cycle arrest
- ionic liquid
- cell death
- insulin resistance
- mass spectrometry