Red blood cell transfusion-induced non-transferrin-bound iron promotes Pseudomonas aeruginosa biofilms in human sera and mortality in catheterized mice.
Francesca La CarpiaAndrea SlateSheila BandyopadhyayBoguslaw S WojczykElizabeth A GodbeyKevin P FrancisKevin PrestiaEldad A HodPublished in: British journal of haematology (2021)
Transfusion of storage-damaged red blood cells (RBCs) increases non-transferrin-bound iron (NTBI) levels in humans. This can potentially enhance virulence of microorganisms. In this study, Pseudomonas aeruginosa replication and biofilm production in vitro correlated with NTBI levels of transfused subjects (R2 = 0·80; P < 0·0001). Transfusion of stored RBCs into catheterized mice enhanced P. aeruginosa virulence and mortality in vivo, while pre-administration of apotransferrin reduced NTBI levels improving survival (69% vs 27% mortality; P < 0·05). These results suggest that longer RBC storage, by modulating the bioavailability of iron, may increase the risk of P. aeruginosa biofilm-related infections in transfused patients.
Keyphrases
- red blood cell
- pseudomonas aeruginosa
- biofilm formation
- cystic fibrosis
- cardiovascular events
- acinetobacter baumannii
- cardiac surgery
- staphylococcus aureus
- endothelial cells
- end stage renal disease
- candida albicans
- high fat diet induced
- newly diagnosed
- risk factors
- ejection fraction
- escherichia coli
- high glucose
- sickle cell disease
- prognostic factors
- chronic kidney disease
- oxidative stress
- drug resistant
- coronary artery disease
- room temperature
- diabetic rats
- patient reported