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Assessing nuclear versus mitochondrial cell-free DNA (cfDNA) by qRT-PCR and droplet digital PCR using a piglet model of perinatal asphyxia.

Marie BitencBenedicte Grebstad TuneMaria MelheimMonica Atneosen-ÅseggXiaoran LaiPolona RajarRønnaug SolbergLars Oliver Baumbusch
Published in: Molecular biology reports (2022)
Both, qRT-PCR and the droplet digital PCR technique identified overall similar patterns for the concentration changes of cfDNA; but, the more sensitive digital PCR methodology might be required to identify minimal responses.
Keyphrases
  • real time pcr
  • high throughput
  • single cell
  • pregnant women