Adrenergic Reprogramming of Preexisting Adipogenic Trajectories Steer Naïve Mural Cells Toward Beige Differentiation.
Kathleen DesevinBriana N CortezJean Z LinDechen LamaMatthew D LayneStephen R FarmerNabil RabhiPublished in: bioRxiv : the preprint server for biology (2023)
In adult white adipose tissue, cold or β3-adrenoceptor activation promotes the appearance of thermogenic beige adipocytes. Our comprehensive single-cell analysis revealed that these cells arise through the reprogramming of existing adipogenic trajectories, rather than from a single precursor. These trajectories predominantly arise from SM22-expressing vascular mural progenitor cells. Central in this transition is the activation of Adrb3 in mature adipocytes, leading to subsequent upregulation of Adrb1 in primed progenitors. Under thermoneutral conditions, synergistic activation of both Adrb3 and Adrb1 recapitulates the pattern of cold-induced SM22+ cell recruitment. Lipolysis-derived eicosanoids, specifically docosahexaenoic acid (DHA) and arachidonic acid (AA) prime these processes and in vitro, were sufficient to recapitulate progenitor cells priming. Collectively, our findings provide a robust model for cold-induced beige adipogenesis, emphasizing a profound relationship between mature adipocytes and mural cells during cold acclimation, and revealing the metabolic potential of this unique cellular reservoir.
Keyphrases
- adipose tissue
- induced apoptosis
- single cell
- cell cycle arrest
- depressive symptoms
- high glucose
- insulin resistance
- cell proliferation
- type diabetes
- rna seq
- cell death
- stem cells
- metabolic syndrome
- diabetic rats
- oxidative stress
- bone marrow
- cell therapy
- drug induced
- endothelial cells
- skeletal muscle
- risk assessment
- high fat diet induced
- climate change
- stress induced