Detection of Antimicrobial Resistance Using Proteomics and the Comprehensive Antibiotic Resistance Database: A Case Study.
Julie Chih-Yu ChenClifford G ClarkStacie LangnerDavid A BoydAmrita BharatStuart J McCorristerAndrew G McArthurMorag R GrahamGarrett R WestmacottGary Van DomselaarPublished in: Proteomics. Clinical applications (2020)
Both genomic and proteomic approaches identify the wild-type and variant molecular determinants responsible for resistance to tetracycline and ciprofloxacin, in agreement with phenotypic testing. In contrast, the genomic method identifies the presence of the β-lactamase gene, blaOXA - 61 , in three isolates. However, its corresponding protein product is detected in only a single isolate, consistent with results obtained from phenotypic testing.
Keyphrases
- antimicrobial resistance
- wild type
- copy number
- label free
- genome wide
- klebsiella pneumoniae
- escherichia coli
- pseudomonas aeruginosa
- acinetobacter baumannii
- mass spectrometry
- multidrug resistant
- protein protein
- gene expression
- single molecule
- emergency department
- loop mediated isothermal amplification
- real time pcr
- contrast enhanced
- magnetic resonance imaging
- computed tomography
- visible light
- sensitive detection