Escherichia coli DNA ligase B may mitigate damage from oxidative stress.
Truston J BodineMichael A EvangelistaHuan Ting ChangChristopher A AyoubBuck S SamuelRichard SucgangLynn ZechiedrichPublished in: PloS one (2017)
Escherichia coli encodes two DNA ligases, ligase A, which is essential under normal laboratory growth conditions, and ligase B, which is not. Here we report potential functions of ligase B. We found that across the entire Enterobacteriaceae family, ligase B is highly conserved in both amino acid identity and synteny with genes associated with oxidative stress. Deletion of ligB sensitized E. coli to specific DNA damaging agents and antibiotics resulted in a weak mutator phenotype, and decreased biofilm formation. Overexpression of ligB caused a dramatic extension of lag phase that eventually resumed normal growth. The ligase function of ligase B was not required to mediate the extended lag phase, as overexpression of a ligase-deficient ligB mutant also blocked growth. Overexpression of ligB during logarithmic growth caused an immediate block of cell growth and DNA replication, and death of about half of cells. These data support a potential role for ligase B in the base excision repair pathway or the mismatch repair pathway.
Keyphrases
- escherichia coli
- oxidative stress
- biofilm formation
- pseudomonas aeruginosa
- circulating tumor
- induced apoptosis
- transcription factor
- cell proliferation
- staphylococcus aureus
- single molecule
- dna damage
- cell free
- amino acid
- machine learning
- risk assessment
- deep learning
- diabetic rats
- signaling pathway
- artificial intelligence
- circulating tumor cells
- data analysis