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Clonal chromosomal and genomic instability during human multipotent mesenchymal stromal cells long-term culture.

Victoria A NikitinaTatiana AstrelinaVladimir NugisAleksandr OstashkinTatiana KarasevaEkaterina DobrovolskayaDariya UsupzhanovaYulia SuchkovaElena LomonosovaSergey RodinVitaliy BrunchukovStanislav Lauk-DubitskiyValentin BrumbergAnastasia MachovaIrina KobzevaAndrey BushmanovAleksandr Samoilov
Published in: PloS one (2018)
The spectrum of spontaneous chromosomal aberrations in MSC long-term cultivation has been described. Clonal chromosomal aberrations have been identified. A clone of cells with tetrasomy 8 has been detected in passage 12 and has reached the maximum size by passage 18 before and decreased along with the reduction of proliferative activity of cell line by passage 26. At later passages, the MSC line exhibited a set of cells with structural variants of the karyotype with a preponderance of normal diploid cells. The results of our study strongly suggest a need for rigorous genetic analyses of the clone formation in cultured MSCs before use in medicine.
Keyphrases
  • copy number
  • induced apoptosis
  • cell cycle arrest
  • endothelial cells
  • bone marrow
  • signaling pathway
  • mesenchymal stem cells
  • gene expression
  • oxidative stress
  • cell proliferation
  • cell death