Calcium-induced differentiation in normal human colonoid cultures: Cell-cell / cell-matrix adhesion, barrier formation and tissue integrity.
Durga AttiliShannon D McClintockAreeba H RizviShailja PandyaHumza RehmanDaniyal M NadeemAliah RichterDafydd ThomasMichael K DameDanielle Kim TurgeonJames VaraniMuhammad N AslamPublished in: PloS one (2019)
These findings demonstrate that colonoids derived from histologically normal human tissue can undergo differentiation in the presence of a low ambient calcium concentration. However, higher calcium levels induce elaboration of proteins that promote cell-cell and cell-matrix adhesion. These changes could lead to improved barrier function and improved colon tissue health.