Design and implementation of a novel pharmacogenetic assay for the identification of the CYP2D6*10 genetic variant.
Nadeeka Dimuthu Kumari RanadevaNirmala Dushyanthi SirisenaTithila Kalum WetthasingheNafeesa NoordeenVajira Harshadeva Weerabaddana DissanayakePublished in: BMC research notes (2022)
The novel assay was successfully designed, optimized and validated using Sanger sequencing. Blood samples from 70 patients were genotyped. The following bands were observed in the gel image: Control band at 454 bp; band for C allele at 195 bp; band for T allele at 300 bp. The genotype frequencies for the CYP2D6*10 (rs1065852C>T) variant were: CC-24.28% (17/70), CT-75.71% (53/70), TT-0% (0/70). The allele frequencies were: T-allele-37.86% and C-allele-62.14%.