Enhanced Tracking of Nosocomial Transmission of Endemic Sequence Type 22 Methicillin-Resistant Staphylococcus aureus Type IV Isolates among Patients and Environmental Sites by Use of Whole-Genome Sequencing.
Peter M KinneveyAnna C ShoreMicheál Mac AogáinEilish CreamerGráinne I BrennanHilary HumphreysThomas R RogersBrian O'ConnellDavid C ColemanPublished in: Journal of clinical microbiology (2015)
Whole-genome sequencing (WGS) of 41 patient and environmental sequence type 22 methicillin-resistant Staphylococcus aureus staphylococcal cassette chromosome mec type IV (ST22-MRSA-IV) isolates recovered over 6 weeks in one acute hospital ward in Dublin, Ireland, where ST22-MRSA IV is endemic, revealed 228 pairwise combinations differing by <40 single nucleotide variants corresponding to potential cross-transmission events (CTEs). In contrast, 15 pairwise combinations of isolates representing five CTEs were previously identified by conventional molecular epidemiological typing. WGS enhanced ST22-MRSA-IV tracking and highlighted potential transmission of MRSA via the hospital environment.
Keyphrases
- methicillin resistant staphylococcus aureus
- staphylococcus aureus
- healthcare
- human health
- genetic diversity
- magnetic resonance
- copy number
- magnetic resonance imaging
- liver failure
- intensive care unit
- risk assessment
- climate change
- hepatitis b virus
- computed tomography
- multidrug resistant
- aortic dissection
- respiratory failure
- extracorporeal membrane oxygenation
- amino acid
- klebsiella pneumoniae