MAxSIM: multi-angle-crossing structured illumination microscopy with height-controlled mirror for 3D topological mapping of live cells.
Pedro Felipe Gardeazabal RodriguezYigal LilachAbhijit AmbegaonkarTeresa VitaliHaani JafriHae Won SohnMatthew B DalvaSusan PierceInhee ChungPublished in: Communications biology (2023)
Mapping 3D plasma membrane topology in live cells can bring unprecedented insights into cell biology. Widefield-based super-resolution methods such as 3D-structured illumination microscopy (3D-SIM) can achieve twice the axial ( ~ 300 nm) and lateral ( ~ 100 nm) resolution of widefield microscopy in real time in live cells. However, twice-resolution enhancement cannot sufficiently visualize nanoscale fine structures of the plasma membrane. Axial interferometry methods including fluorescence light interference contrast microscopy and its derivatives (e.g., scanning angle interference microscopy) can determine nanoscale axial locations of proteins on and near the plasma membrane. Thus, by combining super-resolution lateral imaging of 2D-SIM with axial interferometry, we developed multi-angle-crossing structured illumination microscopy (MAxSIM) to generate multiple incident angles by fast, optoelectronic creation of diffraction patterns. Axial localization accuracy can be enhanced by placing cells on a bottom glass substrate, locating a custom height-controlled mirror (HCM) at a fixed axial position above the glass substrate, and optimizing the height reconstruction algorithm for noisy experimental data. The HCM also enables imaging of both the apical and basal surfaces of a cell. MAxSIM with HCM offers high-fidelity nanoscale 3D topological mapping of cell plasma membranes with near-real-time ( ~ 0.5 Hz) imaging of live cells and 3D single-molecule tracking.
Keyphrases
- high resolution
- single molecule
- induced apoptosis
- high speed
- cell cycle arrest
- atomic force microscopy
- optical coherence tomography
- body mass index
- mass spectrometry
- high throughput
- single cell
- oxidative stress
- signaling pathway
- type diabetes
- endoplasmic reticulum stress
- big data
- magnetic resonance
- minimally invasive
- air pollution
- cardiovascular disease
- mesenchymal stem cells
- deep learning
- cell proliferation
- physical activity
- biofilm formation
- quantum dots
- crystal structure