A simple ex vivo bioassay for 5-FU transport into healthy buccal mucosal cells.

Kathryn Elisa BurnsDaniel AllrightDavid Porter Michael P N Findlay Nuala Ann Helsby

Published in: Cancer chemotherapy and pharmacology (2019)

The uptake of 5-FU into healthy oral mucosal cells is a highly variable process facilitated by membrane transporters at pharmacologically relevant concentrations. This bioassay is simple, minimally invasive, and suitable for phenotypic analysis of drug transport in healthy primary cells.

Keyphrases

induced apoptosis
cell cycle arrest
minimally invasive
endoplasmic reticulum stress
oxidative stress
emergency department
cell death
signaling pathway
robot assisted