Detection of urinary metabolites of arimistane in humans by gas chromatography coupled to high-accuracy mass spectrometry for antidoping analyses.
Dayamin Martinez BritoXavier de la TorreFrancesco BotrePublished in: Rapid communications in mass spectrometry : RCM (2020)
Arimistane itself was not observed in the free or glucuronated fractions, but only in the sulfate fraction. The peaks showing mass spectra in agreement with hydroxylated metabolites did not match with those for 7-keto-DHEA, 7α- or 7β-hydroxy-DHEA. This suggests that the first hydroxylation did not occur on C3, but on C2. These newly described metabolites allow the specific detection of arimistane misuse in sports.
Keyphrases
- gas chromatography
- mass spectrometry
- ms ms
- tandem mass spectrometry
- liquid chromatography
- loop mediated isothermal amplification
- high resolution mass spectrometry
- gas chromatography mass spectrometry
- real time pcr
- label free
- high performance liquid chromatography
- high resolution
- capillary electrophoresis
- density functional theory