Detection of IDH1 Mutation in cfDNA and Tissue of Adult Diffuse Glioma with Allele-Specific qPCR.

Adil HusainSridhar MishraMohammed Haris SiddiquiNuzhat Husain

Published in: Asian Pacific journal of cancer prevention : APJCP (2023)

The CAST-PCR assay is more precise and sensitive for IDH1-R132Hdetection than traditional IHC, and IDH1-R132H mutation detection using cfDNA may add to the current methods of glioma genomic characterization.

Keyphrases

low grade
real time pcr
loop mediated isothermal amplification
wild type
high grade
label free
gene expression
genome wide
dna methylation
childhood cancer