Rational design of thiolated polyenes as trifunctional Raman reporter molecules in surface-enhanced Raman scattering nanotags for cytokine detection in a lateral flow assay.
Thomas KellerSvetlana BremVi TranOliver SritharanDaniel SchäferSebastian SchlückerPublished in: Journal of biophotonics (2020)
The characteristic vibrational spectroscopic fingerprint of Raman reporter molecules adsorbed on noble metal nanoparticles is employed for the identification of target proteins by the corresponding surface-enhanced Raman scattering (SERS) nanotag-labeled antibodies. Here, we present the modular synthesis of thiolated polyenes with two to five C═C double bonds introduced via stepwise Wittig reactions. The experimental characterization of their electronic and vibrational properties is complemented by density functional theory calculations. Highly SERS-active nanotags are generated by using the thiolated polyenes as Raman reporter molecules in Au/Au core/satellite supraparticles with multiple hot spots. The cytokines IL-1β and IFN-γ are detected in a duplex SERS-based lateral flow assay on a nitrocellulose test strip by Raman microscopy. The thiolated polyenes are suitable for use in immuno-SERS applications such as point-of-care testing as well as cellular and tissue imaging.
Keyphrases
- density functional theory
- label free
- raman spectroscopy
- sensitive detection
- molecular dynamics
- crispr cas
- gold nanoparticles
- high throughput
- loop mediated isothermal amplification
- high resolution
- molecular dynamics simulations
- quantum dots
- reduced graphene oxide
- immune response
- molecular docking
- computed tomography
- mass spectrometry
- real time pcr
- high speed
- energy transfer