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Isolation and Characterization of Germline Stem Cells in Protogynous Hermaphroditic Monopterus albus .

Xiaoyun SunBinbin TaoYongxin WangWei HuYuhua Sun
Published in: International journal of molecular sciences (2022)
Germline stem cells (GSCs) are a group of unique adult stem cells in gonads that act as important transmitters for genetic information. Donor GSCs have been used to produce offspring by transplantation in fisheries. In this study, we successfully isolated and enriched GSCs from the ovary, ovotestis, and testis of M onopterus albus , one of the most important breeding freshwater fishes in China. Transcriptome comparison assay suggests that a distinct molecular signature exists in each type of GSC, and that different signaling activities are required for the maintenance of distinct GSCs. Functional analysis shows that fGSCs can successfully colonize and contribute to the germline cell lineage of a host zebrafish gonad after transplantation. Finally, we describe a simple feeder-free method for the isolation and enrichment of GSCs that can contribute to the germline cell lineage of zebrafish embryos and generate the germline chimeras after transplantation.
Keyphrases
  • stem cells
  • cell therapy
  • single cell
  • dna repair
  • rna seq
  • high throughput
  • genome wide
  • gene expression
  • high fat diet
  • healthcare
  • oxidative stress
  • skeletal muscle
  • health information
  • data analysis