Optogenetic Control of Voltage-Gated Calcium Channels.

Guolin MaJindou LiuYuepeng KeXin LiuMinyong LiFen WangGang HanYun HuangYoujun WangYubin Zhou

Published in: Angewandte Chemie (International ed. in English) (2018)

Voltage-gated Ca2+ (CaV ) channels mediate Ca2+ entry into excitable cells to regulate a myriad of cellular events following membrane depolarization. We report the engineering of RGK GTPases, a class of genetically encoded CaV channel modulators, to enable photo-tunable modulation of CaV channel activity in excitable mammalian cells. This optogenetic tool (designated optoRGK) tailored for CaV channels could find broad applications in interrogating a wide range of CaV -mediated physiological processes.

Keyphrases

induced apoptosis
small molecule
cell cycle arrest
oxidative stress
signaling pathway
smoking cessation
cell proliferation
cell death
quantum dots