Immunoglobulin G genetic variation can confound assessment of antibody levels via altered binding to detection reagents.
Ruth A PurcellL Carissa AureliaRobyn EsterbauerLilith F AllenKatherine A BondDeborah A WilliamsonJanine M TrevillyanJason A TrubianoJennifer J JunoAdam K WheatleyMiles P DavenportThi H O NguyenKatherine KedzierskaStephen J KentKevin John SelvaAmy W ChungPublished in: Clinical & translational immunology (2024)
Anti-IgG1 clone 4E3 confounds assessment of antibody responses in clinical cohorts owing to bias towards detection of G1m1,17 IgG1 variants. Validation of anti-Ig clones should include evaluation of binding to relevant antibody variants, particularly as the role of immunogenetics upon humoral immunity is increasingly explored in diverse populations.