Efficient production of isomaltulose using engineered Yarrowia lipolytica strain facilitated by non-yeast signal peptide-mediated cell surface display.

This study provided an efficient way for manufacturing isomaltulose with high space-time yield. This heterogenous signal peptide-mediated cell surface display strategy featured with small fusion tag (~2.2 kDa of SP1), absence of enzyme leakage in fermentation broth, and ample room for optimization, providing a convenient way to construct whole-cell biocatalysts to synthesize other products and broadening the array of molecular toolboxes accessible for engineering Y. lipolytica. This article is protected by copyright. All rights reserved.