Multiplexing detection of IgG against Plasmodium falciparum pregnancy-specific antigens.
Ana Maria FonsecaLlorenç QuintoAlfons JiménezRaquel GonzálezAzucena BardajíSonia MaculuveCarlota DobañoMaria RupérezAnifa ValaJohn J AponteEsperanza SeveneEusebio MaceteClara MenéndezAlfredo Gabriel Mayor AparicioPublished in: PloS one (2017)
The multiplex bead-based immunoassay to detect antibodies against selected 25 VAR2CSA new-peptides and recombinant-domains was successfully implemented. Analysis of field samples showed that responses were specific among pregnant women and dependent on the level of exposure to malaria. This platform provides a high-throughput approach to investigating correlates of protection and identifying serological markers of exposure for malaria in pregnancy.