Cucumis sativus L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway.
Muqeet WahidFatima SaqibHanadi Talal AhmedahClaudia Mihaela GavrisVincenzo De FeoMircea HogeaMarius Alexandru MogaRadu ChiceaPublished in: Pharmaceuticals (Basel, Switzerland) (2021)
Cucumis sativus L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a C. sativus seeds extract against asthma and diarrhea using network pharmacology along with a molecular docking approach. Furthermore, in-vitro and in-vivo experiments were conducted to verify the mechanistic insight of in silico studies. LC-ESI-MS/MS was performed to identify the bioactive compounds in the extract; later, some compounds were quantified by HPLC. C. sativus seed. EtOH has kaempferol in higher concentration 783.02 µg/g, followed by quercetin (693.83 µg/g) and luteolin (617.17 µg/g). In silico studies showed that bioactive compounds interfered with asthma and diarrhea-associated target genes, which are members of calcium-mediated signaling to exert a calcium channel blocker activity. The seeds extract exerted a concentration-dependent spasmolytic response on isolated jejunum, trachea, and urinary bladder preparations and caused relaxation of spastic contraction of K+ (80 mM) with suppressed calcium concentration-response curves at dose 0.3 and 1 mg/mL. It also showed antiperistalsis, antidiarrheal and antisecretory activity in animal models. Thus, C. sativus seeds have therapeutic effects by regulating the contractile response through a calcium-mediated signaling pathway.
Keyphrases
- molecular docking
- ms ms
- signaling pathway
- oxidative stress
- chronic obstructive pulmonary disease
- lung function
- pi k akt
- skeletal muscle
- epithelial mesenchymal transition
- mass spectrometry
- induced apoptosis
- high resolution
- smooth muscle
- diabetic rats
- allergic rhinitis
- cystic fibrosis
- high glucose
- genome wide
- tandem mass spectrometry
- case control
- air pollution
- high performance liquid chromatography
- endoplasmic reticulum stress
- high resolution mass spectrometry
- genome wide identification
- bioinformatics analysis
- network analysis
- gas chromatography