Stepwise phosphorylation of BLT1 defines complex assemblies with β-arrestin serving distinct functions.

G protein-coupled receptors (GPCRs) utilize complex cellular systems to respond to diverse ligand concentrations. By taking BLT1, a GPCR for leukotriene B 4 (LTB 4 ), as a model, our previous work elucidated that this system functions through the modulation of phosphorylation status on two specific residues: Thr 308 and Ser 310 . Ser 310 phosphorylation occurs at a lower LTB 4 concentration than Thr 308 , leading to a shift in ligand affinity from a high-to-low state. However, the implications of BLT1 phosphorylation in signal transduction processes or the underlying mechanisms have remained unclear. Here, we identify the sequential BLT1-engaged conformations of β-arrestin and subsequent alterations in signal transduction. Stimulation of the high-affinity BLT1 with LTB 4 induces phosphorylation at Ser 310 via the ERK1/2-GRK pathway, resulting in a β-arrestin-bound low-affinity state. This configuration, referred to as the "low-LTB 4 -induced complex," necessitates the finger loop region and the phosphoinositide-binding motif of β-arrestins to interact with BLT1 and deactivates the ERK1/2 signaling. Under high LTB 4 concentrations, the low-affinity BLT1 again binds to the ligand and triggers the generation of the low-LTB 4 -induced complex into a different form termed "high-LTB 4 -induced complex." This change is propelled by The 308 -phosphorylation-dependent basal phosphorylation by PKCs. Within the high-LTB 4 -induced complex, β-arrestin adapts a unique configuration that involves additional N domain interaction to the low-affinity BLT1 and stimulates the PI3K/AKT pathway. We propose that the stepwise phosphorylation of BLT1 defines the formation of complex assemblies, wherein β-arrestins perform distinct functions.