Proteomic Analysis and Cell Viability of Nine Amnion, Chorion, Umbilical Cord, and Amniotic Fluid-Derived Products.
Liliya BecktellAndrea M MatuskaStephanie HonMichelle L DelcoBrian J ColeLaila BegumSheng ZhangLisa Ann FortierPublished in: Cartilage (2020)
Several novel proteins were identified through proteomics that might impart the beneficial effects of amnion products, including SLRPs, collagens, and regulators of fibroblast activity. IL-1Ra and TIMP-2 were identified, but concentrations measured by ELISA may be falsely increased due to nonspecific antibody binding. The concept that the amnion tissues provide live cells to aid in tissue regeneration cannot be supported by the findings of this study.
Keyphrases
- umbilical cord
- mesenchymal stem cells
- induced apoptosis
- stem cells
- rheumatoid arthritis
- cell cycle arrest
- gene expression
- mass spectrometry
- transcription factor
- oxidative stress
- bone marrow
- signaling pathway
- disease activity
- cell death
- systemic lupus erythematosus
- ankylosing spondylitis
- dna binding
- idiopathic pulmonary fibrosis