Bioorthogonally applicable multicolor fluorogenic naphthalimide-tetrazine probes with aggregation-induced emission characters.
Yongcheng WangYu TengHong YangXiang LiDali YinYulin TianPublished in: Chemical communications (Cambridge, England) (2022)
A series of naphthalimide - tetrazines were developed as bioorthogonal fluorogenic probes, which could produce significant fluorescence enhancement, notable aggregation-induced emission (AIE) characters and multicolor emissions after bioorthogonal reaction with strained dienophiles. Manipulating the π-bridge in the fluorophore skeleton allows fine-tuning of the emission wavelength and influences the AIE-active properties. With these probes, we succeeded in no-wash fluorogenic protein labeling and mitochondria-selective bioorthogonal imaging in live cells.
Keyphrases
- fluorescent probe
- living cells
- single molecule
- induced apoptosis
- small molecule
- fluorescence imaging
- cell cycle arrest
- solid state
- cell death
- air pollution
- reactive oxygen species
- oxidative stress
- binding protein
- cell proliferation
- amino acid
- signaling pathway
- endoplasmic reticulum stress
- endoplasmic reticulum
- mass spectrometry
- photodynamic therapy
- risk assessment