Bioanalysis of plasma acetate levels without derivatization by LC-MS/MS.
Rani J QasemIbrahim K FrahAhmad S AljadaFaisal A SehliPublished in: Bioanalysis (2021)
Background: The acetate ion has important physiological functions and important therapeutic applications. A rapid LC-MS/MS method is described to measure acetate ions in human plasma without chemical derivatization. Materials & methods: A 200 μl sample was spiked with the internal standard 1,2-13C-acetate and proteins precipitated with trichloroacetic acid. The supernatant was recovered and separated under acidic conditions on a C18-column. The eluent was alkalinized by post-column infusion of methanolic ammonium hydroxide. Acetate ions were monitored on a low resolution mass spectrometer in negative ion mode. Results: Method was validated for accuracy and precision with a lower limit of quantitation of 9.7 μM and linear dynamic range up to 339.6 μM. Conclusion: The method is open for analytical improvement and adapts with metabolomic and pharmacometabolomic studies on chemicals of similar nature.
Keyphrases
- liquid chromatography
- ms ms
- solid phase extraction
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- mass spectrometry
- tandem mass spectrometry
- simultaneous determination
- high resolution mass spectrometry
- gas chromatography mass spectrometry
- quantum dots
- high resolution
- low dose
- minimally invasive
- ionic liquid
- case control