Establishing a Standardized DNA Extraction Method Using NaCl from Oral Mucosa Cells for Its Application in Imprinting Diseases Such as Prader-Willi and Angelman Syndromes: A Preliminary Investigation.
Letícia Lopes Cabral Guimarães da FonsecaDanielle Nascimento RochaHiago Azevedo CintraLuiza Loureiro de AraújoGabrielle Leal Monteiro Dos SantosLeonardo Lima de FariaMargarida Dos Santos SalúSilvia Helena Dos Santos LeiteAdriana Duarte RochaMaria da Conceição Borges LopesIgor Ribeiro FerreiraLeonardo Henrique Ferreira GomesLetícia Cunha GuidaPublished in: Genes (2024)
Our study emphasizes the significance of oral swab samples as a reliable source for obtaining DNA for MS-HRM analysis. NaCl extraction stands out as a practical and cost-effective method for genetic studies, contributing to a molecular diagnosis that proves particularly beneficial for patients facing delays in characterization, ultimately influencing their treatment.