Molecular diagnosis of Cytomegalovirus infection: clinical performance of the Aptima transcription-mediated amplification assay toward conventional qPCR chemistry on whole blood samples.
Paolo BottinoLisa PastroneElisa ZanottoFrancesca SidotiRossana CavalloCristina CostaPublished in: Journal of clinical microbiology (2024)
In this paper, we describe the clinical performance of a novel transcription-mediated amplification (TMA) assay for the detection and quantification of human Cytomegalovirus (HCMV) DNA from whole blood samples. This is a pivotal analysis in immunocompromised patients [transplanted, HIV-positive, and Hematopoietic Stem Cell (HSC) recipients], and molecular tests with high sensitivity and specificity are necessary to evaluate the HCMV viral load in these patients. To our knowledge, this is the first in-depth evaluation of TMA chemistry for HCMV diagnosis on whole blood samples. Moreover, also technical aspects of this assay make it suitable for clinical diagnostics.
Keyphrases
- end stage renal disease
- hiv positive
- ejection fraction
- newly diagnosed
- chronic kidney disease
- high throughput
- peritoneal dialysis
- prognostic factors
- endothelial cells
- hematopoietic stem cell
- transcription factor
- nucleic acid
- south africa
- label free
- patient reported outcomes
- human immunodeficiency virus
- hiv infected
- extracorporeal membrane oxygenation