Ultrabright Fluorescent Organic Nanoparticles Based on Small-Molecule Ionic Isolation Lattices*.
Junsheng ChenS M Ali FateminiaLaura KacenauskaiteNicolai BaerentsenStine Grønfeldt StenspilJona BredehoeftKaren L MartinezAmar H FloodBo W LaursenPublished in: Angewandte Chemie (International ed. in English) (2021)
Ultrabright fluorescent nanoparticles (NPs) hold great promise for demanding bioimaging applications. Recently, extremely bright molecular crystals of cationic fluorophores were obtained by hierarchical coassembly with cyanostar anion-receptor complexes. These small-molecule ionic isolation lattices (SMILES) ensure spatial and electronic isolation to prohibit aggregation quenching of dyes. We report a simple, one-step supramolecular approach to formulate SMILES materials into NPs. Rhodamine-based SMILES NPs stabilized by glycol amphiphiles show high fluorescence quantum yield (30 %) and brightness per volume (5000 M-1 cm-1 /nm3 ) with 400 dye molecules packed into 16-nm particles, corresponding to a particle absorption coefficient of 4×107 M-1 cm-1 . UV excitation of the cyanostar component leads to higher brightness (>6000 M-1 cm-1 / nm3 ) by energy transfer to rhodamine emitters. Coated NPs stain cells and are thus promising for bioimaging.
Keyphrases
- energy transfer
- quantum dots
- small molecule
- fluorescent probe
- living cells
- light emitting
- photodynamic therapy
- ionic liquid
- oxide nanoparticles
- protein protein
- induced apoptosis
- cell cycle arrest
- aqueous solution
- room temperature
- solid state
- computed tomography
- big data
- magnetic resonance imaging
- deep learning
- highly efficient
- binding protein
- cell proliferation
- diffusion weighted imaging