Sensitive and semiquantitative detection of soil-transmitted helminth infection in stool using a recombinase polymerase amplification-based assay.
Jason L CanteraHeather N WhiteMatthew S ForrestOliver W StringerVicente Y BelizarioHelen L StoreyEugenio L de HostosTala de Los SantosPublished in: PLoS neglected tropical diseases (2021)
Semi-quantitation of target by RPA assay is possible and is comparable to real-time PCR. With proper instrumentation, RPA assays can provide robust, semi-quantification of STH DNA targets as an alternative field-deployable indicator to counts of helminth eggs for assessing infection intensity.
Keyphrases
- real time pcr
- high throughput
- nucleic acid
- ms ms
- mass spectrometry
- circulating tumor
- label free
- high intensity
- liquid chromatography tandem mass spectrometry
- cell free
- peripheral blood
- tandem mass spectrometry
- loop mediated isothermal amplification
- high performance liquid chromatography
- single cell
- high resolution
- circulating tumor cells
- simultaneous determination