A proof-of-principle study for the point-of-care detection of ESBL (CTX-M) by NG-Test ® CTX-M MULTI lateral flow assay in urine samples using a simplified method for use in a resource-limited setting.

Our study underscores the feasibility of directly detecting CTX-M in urine. Simplified enrichment methods, particularly with a filtration kit, enhance the assay's practicality, rendering it suitable for use in primary care, emergency departments or remote laboratories without sophisticated equipment.