Molecular basis of carrageenan-induced cytokines production in macrophages.
Alexandre H LopesRangel L SilvaMiriam D FonsecaFrancisco I GomesAlexandre G MaganinLucas S RibeiroLucas Maciel Mauriz MarquesFernando Q CunhaJose C Alves-FilhoDario S ZamboniNorberto P LopesBernardo S FranklinAurélie GombaultFernando Silva RamalhoValerie F J QuesniauxIsabelle CouillinBernhard RyffelThiago Mattar CunhaPublished in: Cell communication and signaling : CCS (2020)
In conclusion, we unravel a critical role of the NLRP3 inflammasome in Cg-induced pro-inflammatory cytokines production and colitis, which is an important discovery on the pro-inflammatory properties of this sulfated polysaccharide for pre-clinical studies. Video abstract Carrageenan (Cg) is one the most used flogistic stimulus in preclinical studies. Nevertheless, the molecular basis of Cg-induced inflammation is not totally elucidated. Herein, Lopes et al. unraveled the molecular basis for Cg-induced macrophages production of biological active IL-1β. The Cg-stimulated macrophages produces pro-IL-1β depends on TLR4/TRIF/Syk/ROS, whereas its processing into mature IL-1β is dependent on the canonical NLRP3 inflammasome.