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A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy.

Gabriela MatelaPeng GaoGernot GuigasAntonia F EckertKarin NienhausGerd Ulrich Nienhaus
Published in: Chemical communications (Cambridge, England) (2018)
Here we present mGarnet2, a monomeric, far-red fluorescent marker protein derived from mRuby, with absorption and emission bands peaking at 598 and 671 nm, respectively. The protein shows excellent performance as a live-cell fusion marker for STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.
Keyphrases
  • quantum dots
  • photodynamic therapy
  • protein protein
  • living cells
  • label free
  • small molecule
  • high throughput
  • optical coherence tomography
  • energy transfer