Discovery and Heterologous Biosynthesis of the Burnettramic Acids: Rare PKS-NRPS-Derived Bolaamphiphilic Pyrrolizidinediones from an Australian Fungus, Aspergillus burnettii.

Hang LiCameron L M GilchristHeather J LaceyAndrew CrombieDaniel VuongJohn I PittErnest LaceyYit Heng Chooi Andrew M Piggott

Published in: Organic letters (2019)

The burnettramic acids are a new class of antibiotics from an Australian fungus Aspergillus burnettii. The rare bolaamphiphilic scaffold consists of β-d-mannose linked to a pyrrolizidinedione unit via a 26-carbon chain. The most abundant metabolite displayed potent in vitro antifungal activity. Comparative genomics identified the hybrid PKS-NRPS bua gene cluster, which was verified by heterologous pathway reconstitution in Aspergillus nidulans.

Keyphrases

cell wall
small molecule
single cell
saccharomyces cerevisiae
genome wide
gene expression
high throughput