ZnO Nanoparticles-Modified Dressings to Inhibit Wound Pathogens.
Sajjad Mohsin I RayyifHamzah Basil MohammedCarmen CuruțiuAlexandra Cătălina BîrcăAlexandru-Mihai GrumezescuBogdan Ștefan VasileLia Mara DițuVeronica LazărMariana Carmen ChifiriucGrigore MihăescuAlina Maria HolbanPublished in: Materials (Basel, Switzerland) (2021)
Zinc oxide (ZnO) nanoparticles (NPs) have been investigated for various skin therapies in recent years. These NPs can improve the healing and modulate inflammation in the wounds, but the mechanisms involved in such changes are yet to be known. In this study, we have designed a facile ZnO nano-coated dressing with improved antimicrobial efficiency against typical wound pathogens involved in biofilm and chronic infections. ZnO NPs were obtained by hydrothermal method and characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Fourier-transform infrared spectroscopy. Antibacterial and antibiofilm effects were evaluated against laboratory and clinical isolates of significant Gram-negative (Pseudomonas aeruginosa and Escherichia coli) and Gram-positive (Staphylococcus aureus and Enterococcus faecalis) opportunistic pathogens, by quantitative methods. Our results have shown that the developed dressings have a high antibacterial efficiency after 6-24 h of contact when containing 0.6 and 0.9% ZnO NPs and this effect is similar against reference and clinical isolates. Moreover, biofilm development is significantly impaired for up to three days of contact, depending on the NPs load and microbial species. These results show that ZnO-coated dressings prevent biofilm development of main wound pathogens and represent efficient candidates for developing bioactive dressings to fight chronic wounds.
Keyphrases
- gram negative
- electron microscopy
- staphylococcus aureus
- pseudomonas aeruginosa
- quantum dots
- multidrug resistant
- room temperature
- wound healing
- reduced graphene oxide
- biofilm formation
- oxide nanoparticles
- visible light
- acinetobacter baumannii
- candida albicans
- cystic fibrosis
- high resolution
- methicillin resistant staphylococcus aureus
- drug resistant
- surgical site infection
- antimicrobial resistance
- oxidative stress
- drug induced
- gold nanoparticles
- ionic liquid
- silver nanoparticles
- anti inflammatory
- magnetic resonance imaging