pH-Induced reorientation of cytochrome c on silica nanoparticles.

The orientation of cytochrome c molecules at the surface of silica nanoparticles was studied in a wide pH range by combining small-angle neutron scattering, adsorption measurements, and molecular dynamics simulations. The results indicate a reorientation of the ellipsoidal protein from head-on to side-on as the pH is increased. This is attributed to changes in the surface charge distribution of both the protein and the nanoparticles.