Determining the cytotoxicity of the Minimum Inhibitory Concentration (MIC) of silver and zinc oxide nanoparticles in ESBL and carbapenemase producing Proteus mirabilis isolated from clinical samples in Shiraz, Southwest Iran.
Farshad KakianEsmaeil MirzaeiAfagh MoattariSara TakalluAbdollah BazarganiPublished in: BMC research notes (2024)
A total of 100 P. mirabilis isolates were screened by biochemical tests and polymerase chain reaction (PCR). Also, 25 (25%) and 7 (7%) isolates were positive for extended-spectrum beta-lactamase (ESBLs) and carbapenemase, respectively. Synthesized nanoparticles were characterized by UV-vis spectrum, X-ray diffraction (XRD), and electron microscopy. The average size of AgNPs and ZnONPs in the present study is 48 and < 70 nm, respectively. The MIC and the MBC of the ZnONPs were in the range of 31.25 µg/ml and 62.5 µg/mL, respectively. Also, for AgNPs, the MIC and the MBC were in the range of 7.8 µg/mL and 15.6 µg/mL, respectively. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in a primary culture of fibroblast L929 cells for this MIC indicated biocompatibility and low cytotoxicity of Ag NPs and for ZnONPs indicated significant cytotoxicity. Also, a MIC of AgNPs can be used as a therapeutic concentration without the effect of cytotoxicity in human cells.
Keyphrases
- oxide nanoparticles
- klebsiella pneumoniae
- electron microscopy
- silver nanoparticles
- escherichia coli
- gram negative
- multidrug resistant
- acinetobacter baumannii
- high throughput
- genetic diversity
- magnetic resonance imaging
- photodynamic therapy
- cell cycle arrest
- cystic fibrosis
- oxidative stress
- pseudomonas aeruginosa
- signaling pathway
- single cell
- real time pcr
- walled carbon nanotubes