Defined serum-free culture of human infant small intestinal organoids with predetermined doses of Wnt3a and R-spondin1 from surgical specimens.

Yuka MatsumotoHiroyuki KogaMirei TakahashiKazuto SudaTakanori OchiShogo SeoGo MiyanoYuichiro MiyakeHideaki NakajimaShiho YoshidaTakafumi MikamiTadaharu OkazakiNobutaka HattoriAtsuyuki YamatakaTetsuya Nakamura

Published in: Pediatric surgery international (2021)

SI cells can be grown as organoids under defined conditions. This could provide a reproducible and customizable method of using surgical specimens for the study of intestinal maturation and their relevance to pediatric diseases.

Keyphrases

induced pluripotent stem cells
induced apoptosis
endothelial cells
stem cells
cell cycle arrest
cell proliferation
childhood cancer
plant growth