Intracellular distribution and stability of a luminescent rhenium(i) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging.
J L WeddingHugh H HarrisC A BaderS E PlushR MakM MassiD A BrooksB LaiS VogtM V WerrettP V SimpsonB W SkeltonS StagniPublished in: Metallomics : integrated biometal science (2017)
Optical epifluorescence microscopy was used in conjunction with X-ray fluorescence imaging to monitor the stability and intracellular distribution of the luminescent rhenium(i) complex fac-[Re(CO)3(phen)L], where phen = 1,10-phenathroline and L = 5-(4-iodophenyl)tetrazolato, in 22Rv1 cells. The rhenium complex showed no signs of ancillary ligand dissociation, a conclusion based on data obtained via X-ray fluorescence imaging aligning iodine and rhenium distributions. A diffuse reticular localisation was detected for the complex in the nuclear/perinuclear region of cells, by either optical or X-ray fluorescence imaging techniques. X-ray fluorescence also showed that the rhenium complex disrupted the homeostasis of some biologically relevant elements, such as chlorine, potassium and zinc.
Keyphrases
- fluorescence imaging
- high resolution
- photodynamic therapy
- dual energy
- induced apoptosis
- high speed
- computed tomography
- cell cycle arrest
- mycobacterium tuberculosis
- quantum dots
- mass spectrometry
- sensitive detection
- signaling pathway
- energy transfer
- cell death
- high throughput
- magnetic resonance
- drinking water
- resting state
- functional connectivity
- reactive oxygen species
- deep learning
- label free
- contrast enhanced
- data analysis