Effects of lipopolysaccharide on proliferation, migration and osteogenic differentiation of apical papilla cells from early and late stage of root development.
Panuroot AguilarRangsini MahanondaNoppadol Sa-Ard-IamVeera LertchirakarnPublished in: Australian endodontic journal : the journal of the Australian Society of Endodontology Inc (2020)
The aim of this study was to investigate the effects of lipopolysaccharide on cell proliferation, migration and osteogenic differentiation of apical papilla cells from early and late stage of root development. After challenging with various lipopolysaccharide concentrations to apical papilla cells from both stages of root development for 168 h, cell proliferation and migration were investigated. Osteogenic differentiation was examined by Alizarin red staining, and gene expressions of bone/cementum or dentin-related genes were examined by polymerase chain reaction. Lipopolysaccharide did not affect cell proliferation and migration in both groups. Lipopolysaccharide at 1 and 5 µg mL-1 increased Alizarin red staining in apical papilla cells from early-stage but not the late-stage cells. Bone sialoprotein (bone/cementum marker) gene expression increased in both early and late stage of root development at 5 µg mL-1 . These results might explain bone/cementum generation in regenerative endodontic procedures.
Keyphrases
- toll like receptor
- inflammatory response
- gene expression
- bone mineral density
- lps induced
- early stage
- cell proliferation
- mesenchymal stem cells
- cell therapy
- stem cells
- single cell
- bone loss
- squamous cell carcinoma
- induced apoptosis
- bone marrow
- mass spectrometry
- postmenopausal women
- body composition
- oxidative stress
- high resolution
- pi k akt
- cell cycle arrest
- single molecule
- high speed
- locally advanced