Pilea notata ( Pilea notata C. H. Wright_C. H. Wright, 1899) is Pilea Lindl . of Urticaceae, which is a commonly used Miao medicine in Guizhou province. The P. notata chloroplast genome is 150,979 bp, contains a pair of inverted repeats (IRs 25,743bp), and is separated by a large single-copy region (81,446bp) and a small single-copy region (18,047bp). A total of 131 genes, including 86 protein-coding genes, 37 tRNA genes, and eight rRNA genes. Phylogenetic analysis showed that P. notata , P. verrucosa and P. monilifera united as a single branch, while Pilea cadierei was defined as a sister group of this branch.