Stability of Reference Genes for Messenger RNA Quantification by Real-Time PCR in Mouse Dextran Sodium Sulfate Experimental Colitis.

Nour EissaHayam HusseinHongxing WangMohammad F RabbiCharles N BernsteinJean-Eric Ghia

Published in: PloS one (2016)

This study highlights the appropriate choice of RGE to ensure adequate normalization of RT-qPCR data when using this model. Suboptimal RGE may explain controversial results from published studies. We recommend using Tbp and Eef2 instead of Gapdh, Actb or β2m as reference genes.

Keyphrases

real time pcr
genome wide
electronic health record
big data
systematic review
dna methylation
genome wide analysis
decision making
case control
meta analyses