Monocytic and granulocytic myeloid derived suppressor cells differentially regulate spatiotemporal tumour plasticity during metastatic cascade.
Maria OuzounovaEunmi LeeRaziye PiranliogluAbdeljabar El AndaloussiRavindra KolheMehmet F DemirciDaniela MarascoIskander AsmAhmed ChadliKhaled A HassanMuthusamy ThangarajuGang ZhouAli S ArbabJohn K CowellHasan KorkayaPublished in: Nature communications (2017)
It is widely accepted that dynamic and reversible tumour cell plasticity is required for metastasis, however, in vivo steps and molecular mechanisms are poorly elucidated. We demonstrate here that monocytic (mMDSC) and granulocytic (gMDSC) subsets of myeloid-derived suppressor cells infiltrate in the primary tumour and distant organs with different time kinetics and regulate spatiotemporal tumour plasticity. Using co-culture experiments and mouse transcriptome analyses in syngeneic mouse models, we provide evidence that tumour-infiltrated mMDSCs facilitate tumour cell dissemination from the primary site by inducing EMT/CSC phenotype. In contrast, pulmonary gMDSC infiltrates support the metastatic growth by reverting EMT/CSC phenotype and promoting tumour cell proliferation. Furthermore, lung-derived gMDSCs isolated from tumour-bearing animals enhance metastatic growth of already disseminated tumour cells. MDSC-induced 'metastatic gene signature' derived from murine syngeneic model predicts poor patient survival in the majority of human solid tumours. Thus spatiotemporal MDSC infiltration may have clinical implications in tumour progression.
Keyphrases
- induced apoptosis
- squamous cell carcinoma
- small cell lung cancer
- cell proliferation
- single cell
- cell cycle arrest
- gene expression
- epithelial mesenchymal transition
- dna methylation
- endothelial cells
- oxidative stress
- cell therapy
- magnetic resonance imaging
- stem cells
- signaling pathway
- case report
- computed tomography
- peripheral blood
- rna seq
- high glucose
- induced pluripotent stem cells
- free survival